Journal: F1000Research

Article Title: Isolation and characterization of a novel Sphingobium yanoikuyae strain variant that uses biohazardous saturated hydrocarbons and aromatic compounds as sole carbon sources

doi: 10.12688/f1000research.25284.1

Figure Lengend Snippet: ( A ) Chlamydomonas strain 4A+ and CC4533 strain streaked on TAP-agar plate containing 50 µg of polymyxin B/mL of medium. ( B ) Chlamydomonas strain 4A+ and CC4533 strain streaked on TAP-agar plate containing 100 µg polymyxin B/mL of medium. ( C ) Chlamydomonas strain 4A+ and CC4533 strain TAP-agar plate containing 50 µg of neomycin/mL of medium. ( D ) Chlamydomonas strain 4A+ and CC4533 strain streaked on TAP-agar plate containing 100 µg neomycin/mL of medium. TAP-agar antibiotic plates were incubated at room temperature for 2.5 weeks before they were imaged.

Article Snippet: 3) Testing CC4533’s ability to secrete hemolysins : Growth was monitored at 30°C on tryptic soy agar medium plates containing 5% sheep agar (Carolina Biological; Burlington, NC) for a period of 72 hours.

Techniques: Incubation

Journal: F1000Research

Article Title: Isolation and characterization of a novel Sphingobium yanoikuyae strain variant that uses biohazardous saturated hydrocarbons and aromatic compounds as sole carbon sources

doi: 10.12688/f1000research.25284.1

Figure Lengend Snippet: ( A ) Growth on LB-agar medium plate at room temperature (22°C). ( B ) Growth on TAP-agar medium plate at 22°C. ( C ) Growth on LB-agar medium plate at 30°C. ( D ) Growth on TAP-agar medium plate at 30°C. ( E ) Growth on LB-agar medium plate at 37°C. ( F ) Growth on TAP-agar medium plate at 37°C. Culture plates were imaged after 5 days of growth.

Article Snippet: 3) Testing CC4533’s ability to secrete hemolysins : Growth was monitored at 30°C on tryptic soy agar medium plates containing 5% sheep agar (Carolina Biological; Burlington, NC) for a period of 72 hours.

Techniques:

Journal: F1000Research

Article Title: Isolation and characterization of a novel Sphingobium yanoikuyae strain variant that uses biohazardous saturated hydrocarbons and aromatic compounds as sole carbon sources

doi: 10.12688/f1000research.25284.1

Figure Lengend Snippet: ( A ) Control TP + 1% glucose agar medium plate with phenol red as a pH indicator. ( B ) CC4533 on TP +1% glucose agar medium plate with phenol red as a pH indicator. ( C ) Control TP +1% sucrose agar medium plate with phenol red as a pH indicator. ( D ) CC4533 growth on TP +1% sucrose agar medium plate with phenol red as a pH indicator. ( E ) Control TP +1% lactose agar medium plate with phenol red as a pH indicator. ( F ) CC4533 growth on TP +1% lactose agar medium plate with phenol red as a pH indicator. Culture plates were imaged after 5 days of growth at room temperature. CC4533 fermented glucose, sucrose and lactose as evident from the yellow color of phenol red.

Article Snippet: 3) Testing CC4533’s ability to secrete hemolysins : Growth was monitored at 30°C on tryptic soy agar medium plates containing 5% sheep agar (Carolina Biological; Burlington, NC) for a period of 72 hours.

Techniques: Control

Journal: F1000Research

Article Title: Isolation and characterization of a novel Sphingobium yanoikuyae strain variant that uses biohazardous saturated hydrocarbons and aromatic compounds as sole carbon sources

doi: 10.12688/f1000research.25284.1

Figure Lengend Snippet: ( A ) 72 hours-growth of CC4533 on MacConkey Agar medium plate. (B ) 72 hours growth of Escherichia coli on MacConkey Agar medium plate. CC4533 fails to grow on MacConkey agar medium plate. E. coli appears pinkish because it ferments lactose to acid, which causes the neutral red pH indicator to turn red. The dark opaque pink haze on the medium around the E. coli growth is the bile precipitation in acidic environment. Media plates were incubated at 30°C.

Article Snippet: 3) Testing CC4533’s ability to secrete hemolysins : Growth was monitored at 30°C on tryptic soy agar medium plates containing 5% sheep agar (Carolina Biological; Burlington, NC) for a period of 72 hours.

Techniques: Incubation

Journal: F1000Research

Article Title: Isolation and characterization of a novel Sphingobium yanoikuyae strain variant that uses biohazardous saturated hydrocarbons and aromatic compounds as sole carbon sources

doi: 10.12688/f1000research.25284.1

Figure Lengend Snippet: ( A ) 72 hours-growth of CC4533 on Mannitol Salt Agar medium plate. ( B ) 72 hours growth of Staphylococcus aureus on Mannitol Salt Agar medium plate. CC4533 fails to grow on Mannitol Salt Agar medium plate. S. aureus grows on Mannitol Salt Agar and can ferment mannitol. Media plates were incubated at 30°C.

Article Snippet: 3) Testing CC4533’s ability to secrete hemolysins : Growth was monitored at 30°C on tryptic soy agar medium plates containing 5% sheep agar (Carolina Biological; Burlington, NC) for a period of 72 hours.

Techniques: Incubation

Journal: F1000Research

Article Title: Isolation and characterization of a novel Sphingobium yanoikuyae strain variant that uses biohazardous saturated hydrocarbons and aromatic compounds as sole carbon sources

doi: 10.12688/f1000research.25284.1

Figure Lengend Snippet: ( A ) 72-hours growth of CC4533 on tryptic soy-blood agar plate. ( B ) 72 hours growth of Staphylococcus aureus on tryptic soy-blood agar plate. Media plates were incubated at 30°C. Images of 24 hours- and 48-hours growth of both strains are available as Underlying data .

Article Snippet: 3) Testing CC4533’s ability to secrete hemolysins : Growth was monitored at 30°C on tryptic soy agar medium plates containing 5% sheep agar (Carolina Biological; Burlington, NC) for a period of 72 hours.

Techniques: Incubation

Journal: F1000Research

Article Title: Isolation and characterization of a novel Sphingobium yanoikuyae strain variant that uses biohazardous saturated hydrocarbons and aromatic compounds as sole carbon sources

doi: 10.12688/f1000research.25284.1

Figure Lengend Snippet: T ris- P hosphate (TP) agar medium plates shown in & were coated with either cyclohexyl chloride or polyhydroxybutyrate. CC4533 was streaked on the negative control TP-agar medium plate and on the hydrocarbon-coated TP-agar medium plates. After 2 weeks of growth at room temperature, media plates were imaged. ( A ) TP-agar medium plate streaked with CC4533. CC4533 does not grow on TP medium as it lacks a carbon source. ( B ) CC4533 growth on TP-agar medium plate coated with 4 mL of 1% cyclohexyl chloride diluted with chloroform. ( C ) CC4533 growth on TP-agar medium plate coated with 4 mL of 1% polyhydroxybutyrate.

Article Snippet: 3) Testing CC4533’s ability to secrete hemolysins : Growth was monitored at 30°C on tryptic soy agar medium plates containing 5% sheep agar (Carolina Biological; Burlington, NC) for a period of 72 hours.

Techniques: Negative Control

Journal: F1000Research

Article Title: Isolation and characterization of a novel Sphingobium yanoikuyae strain variant that uses biohazardous saturated hydrocarbons and aromatic compounds as sole carbon sources

doi: 10.12688/f1000research.25284.1

Figure Lengend Snippet: T ris- P hosphate (TP) agar medium plates shown in were coated with different does of fresh and combusted car motor oil. CC4533 was streaked on the motor oil-coated TP-agar media plates shown in . After 2 weeks of growth at room temperature, media plates were imaged. ( A ) CC4533 growth on TP-agar medium plate coated with 0.5 mL of 2% fresh 10W-30 car motor oil. ( B ) CC4533 growth on TP-agar medium plate coated with 2 mL of 2% fresh 10W-30 car motor oil. ( C ) CC4533 growth on TP-agar medium plate coated with 0.5 mL of 2% combusted 10W-30 car motor oil. ( D ) CC4533 growth on TP-agar medium plate coated with 2 mL of 2% combusted 10W-30 car motor oil. TP-agar medium plate (lacks a carbon source) shown in served as the negative control for this experiment. Images of plates with different doses of car motor oil are available as Underlying data .

Article Snippet: 3) Testing CC4533’s ability to secrete hemolysins : Growth was monitored at 30°C on tryptic soy agar medium plates containing 5% sheep agar (Carolina Biological; Burlington, NC) for a period of 72 hours.

Techniques: Negative Control

Journal: F1000Research

Article Title: Isolation and characterization of a novel Sphingobium yanoikuyae strain variant that uses biohazardous saturated hydrocarbons and aromatic compounds as sole carbon sources

doi: 10.12688/f1000research.25284.1

Figure Lengend Snippet: TP medium plates shown in were coated with polycyclic aromatic hydrocarbons (PAH). CC4533 was streaked on the negative control TP-agar medium plate and on the PAH-coated TP-agar medium plates. After 2 weeks of growth at room temperature, media plates were imaged. ( A ) TP control plate streaked with CC4533. CC4533 does not grow on TP plate as it lacks a carbon source. ( B ) CC4533 growth on TP plate coated with 4 mL of 1% phenanthrene dissolved in chloroform. ( C ) CC4533 growth on TP plate coated with 0.5 mL of 1% naphthalene dissolved in chloroform. ( D ) CC4533 growth on TP plate coated with 2 mL of 1% naphthalene dissolved in chloroform. Images of plates with different doses of aromatic hydrocarbons are available as Underlying data .

Article Snippet: 3) Testing CC4533’s ability to secrete hemolysins : Growth was monitored at 30°C on tryptic soy agar medium plates containing 5% sheep agar (Carolina Biological; Burlington, NC) for a period of 72 hours.

Techniques: Negative Control, Control

Journal: F1000Research

Article Title: Isolation and characterization of a novel Sphingobium yanoikuyae strain variant that uses biohazardous saturated hydrocarbons and aromatic compounds as sole carbon sources

doi: 10.12688/f1000research.25284.1

Figure Lengend Snippet: TP medium plates shown in were coated with aromatic compounds. CC4533 was streaked on the aromatic compound-coated TP-agar medium plates. After 2 weeks of growth at room temperature, media plates were imaged. ( A ) CC4533 growth on TP plate coated with 0.5 mL of 1% benzoate dissolved in chloroform. ( B ) CC4533 growth on TP plate coated with 2 mL of 1% benzoate dissolved in chloroform. ( C ) CC4533 growth on TP plate coated with 0.5 mL of 1% phenyl acetate dissolved in chloroform. ( D ) CC4533 growth on TP plate coated with 2 mL of 1% phenyl acetate dissolved in chloroform. TP-agar medium plate (lacks a carbon source) shown in ( served as the negative control for this experiment. Images of plates with different doses of aromatic compounds are available as Underlying data .

Article Snippet: 3) Testing CC4533’s ability to secrete hemolysins : Growth was monitored at 30°C on tryptic soy agar medium plates containing 5% sheep agar (Carolina Biological; Burlington, NC) for a period of 72 hours.

Techniques: Negative Control